11 DESCRIPTION
Filgrastim-aafi is a 175 amino acid human granulocyte colony-stimulating factor (G-CSF) manufactured by recombinant DNA technology.
NIVESTYM is produced by Escherichia coli (E coli) bacteria into which has been inserted the human granulocyte colony-stimulating factor gene. NIVESTYM has a molecular weight of 18‚799 daltons. The protein has an amino acid sequence that is identical to the natural sequence predicted from human DNA sequence analysis‚ except for the addition of an N-terminal methionine necessary for expression in E coli. Because NIVESTYM is produced in E coli‚ the product is non-glycosylated and thus differs from G-CSF isolated from a human cell.
NIVESTYM is a sterile‚ clear‚ colorless‚ preservative-free liquid containing filgrastim-aafi injection for subcutaneous or intravenous use. The product is available in single-dose vials and prefilled syringes. The single-dose vials contain either 300 mcg/mL or 480 mcg/1.6 mL of filgrastim-aafi. The single-dose prefilled syringes contain either 300 mcg/0.5 mL or 480 mcg/0.8 mL of filgrastim-aafi. See Table 4 below for product composition of each single-dose vial or prefilled syringe.
| 300mcg/mL Vial | 480 mcg/1.6 mL Vial | 300 mcg/0.5 mL Syringe | 480 mcg/0.8 mL Syringe | |
|---|---|---|---|---|
| ||||
Filgrastim-aafi | 300 mcg | 480 mcg | 300 mcg | 480 mcg |
Acetate | 0.59 mg | 0.94 mg | 0.295 mg | 0.472 mg |
Polysorbate 80 | 0.04 mg | 0.064 mg | 0.02 mg | 0.032 mg |
Sodium | 0.035 mg | 0.056 mg | 0.0175 mg | 0.028 mg |
Sorbitol | 50 mg | 80 mg | 25 mg | 40 mg |
Water for Injection USP | 1 mL | 1.6 mL | 0.5 mL | 0.8 mL |